|
Application Number
|
201910028294
|
Application Date
|
2019.01.11
|
|
Publication Number
|
109504808A
|
Publication Date
|
2019.03.22
|
|
Priority Information
|
|
|
International
Classification
|
C12Q1/70;C12Q1/686;C12N15/11;C12R1/93
|
|
|
Applicant(s) Name
|
INSPECTION & QUARANTINE TECHNOLOGY CENTER OF FUJIAN ENTRY-EXIT INSPECTION & QUARANTINE BUREAU
|
|
|
Address
|
|
|
|
Inventor(s) Name
|
ZHANG TIYIN;LI DANDAN;QIU XIANGGUO;ZHENG TENG;WANG WUJUN;ZHANG ZHIDENG;LIN SUJIE
|
|
|
Patent Agency Code
|
35100
|
Patent Agent
|
Cai Xuejun
|
|
AbstractThe invention provides a group of real-time fluorescent RT-PCR specific primers and probe special for detecting MARV (Marburg virus) and a corresponding detection method established after a reaction system and a reaction program are optimized by the primers and the probe. The real-time fluorescent RT-PCR specific primers and probe and the detection method have the following advantages: (1) good stability and specificity: the primers and the probe have high specificity for detection of the MARV, have no cross reaction with other monkey viruses and have good repeatability; (2) high sensitivity:sensitivity can reach 6.04 copies/mu L; (3) simple and rapid operation: the whole reaction can be completed within 90 min. A kit can be used for detection of the MARV and differential diagnosis of theMARV with other money virus diseases and has great significance and practical application value for ensuring life and health safety of people and animals in China and preventing serious foreign diseases.
|
|